Confocal fluorescence spectroscopy and anisotropy imaging system.
Opt Lett
; 28(9): 695-7, 2003 May 01.
Article
em En
| MEDLINE
| ID: mdl-12747710
ABSTRACT
We report the design and implementation of a laser scanning confocal fluorescence system with spectroscopy and anisotropy imaging capabilities. Confocal spectroscopy is achieved with a fiber pinhole that is inserted into and removed from the detection path as needed. Fluorescence anisotropy imaging is accomplished with a polarizing beam splitter placed after the conventional pinhole. Two orthogonal polarizations are detected simultaneously with balanced photomultiplier tubes. The quality of the axial sectioning that is achieved in the confocal fluorescence spectroscopy mode is demonstrated experimentally, and examples of polarization-sensitive fluorescence imaging are demonstrated in tumor cell monolayers.
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Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Fluorescência
/
Microscopia Confocal
/
Polarização de Fluorescência
Limite:
Animals
Idioma:
En
Revista:
Opt Lett
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Estados Unidos