An inter- and intra-laboratory comparison of breath ¹³CO2analysis.
Aliment Pharmacol Ther
; 17(10): 1291-7, 2003 May 15.
Article
em En
| MEDLINE
| ID: mdl-12755842
ABSTRACT
BACKGROUND:
¹³C breath test analysis requires accurate ¹³CO2measurements.AIM:
To perform a multicentre study to evaluate the repeatability and reproducibility of breath ¹³CO2analysis.METHODS:
Two series of 25 paired randomly coded tubes (each consisting of 23 ¹³CO2-enriched breath samples and two samples of standard reference pure CO2with certified δ ¹³C(PDB)) were sent to participating centres for ¹³CO2measurement. Each series of tubes was analysed 10 days apart. The repeatability and reproducibility of ¹³C measurements was assessed by Mandel's k and h statistics.RESULTS:
Twenty-two centres participated in the study 18 showed good inter- and intra-laboratory variability, whilst four showed abnormally high inter- or intra-laboratory variability. Breath test results were also significantly affected by the accuracy of the ¹³C analytical procedures.CONCLUSIONS:
A low accuracy of ¹³C measurements may significantly affect the results of breath tests, leading to inappropriate clinical decisions. Standardization of ¹³C analysis is required to guarantee optimal ¹³C measurements and accurate ¹³C breath test results.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Testes Respiratórios
/
Dióxido de Carbono
/
Técnicas de Laboratório Clínico
/
Laboratórios
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Humans
Idioma:
En
Revista:
Aliment Pharmacol Ther
Assunto da revista:
FARMACOLOGIA
/
GASTROENTEROLOGIA
/
TERAPIA POR MEDICAMENTOS
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Itália