Cloning of hTERT cDNA fragment and application of anti-hTERT monoclonal antibody in mechanism of laryngeal carcinogenesis.
Yi Chuan Xue Bao
; 30(2): 109-13, 2003 Feb.
Article
em En
| MEDLINE
| ID: mdl-12776597
ABSTRACT
The mechanism of telomerase activation is still not clear till now. In order to understand the expressional mode of telomerase and the mechanism of telomerase activation in laryngeal carcinogenesis, we cloned a fragment of hTERT cDNA and prepared a monoclonal antibody against hTERT. We performed immuno-histochemical staining in laryngeal cancer tissues using this antibody. We found that the frequencies of hTERT positive cells were positively correlated with undifferentiation of cancer tissues and that the expression of hTERT was positively correlated with levels of c-Myc, which suggested that c-Myc might play an important role in activation of telomerase. These results revealed that overexpression of c-Myc upregulated telomerase, which in turn resulted in immortalization of laryngeal squamous cells, and this mechanism existed not only in the initiation of laryngeal carcinogenesis but also in the whole process of cancer development.
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Base de dados:
MEDLINE
Assunto principal:
Neoplasias Laríngeas
/
DNA Complementar
/
Telomerase
/
Anticorpos Monoclonais
Limite:
Humans
Idioma:
En
Revista:
Yi Chuan Xue Bao
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
China