A resource of mapped dissociation launch pads for targeted insertional mutagenesis in the Arabidopsis genome.
Plant Physiol
; 132(2): 506-16, 2003 Jun.
Article
em En
| MEDLINE
| ID: mdl-12805583
ABSTRACT
We describe a new resource for targeted insertional mutagenesis in Arabidopsis using a maize (Zea mays) Activator/Dissociation (Ds) two-element system. The two components of the system, T-DNA vectors carrying a Ds launch pad and a stable Activator transposase source, were designed to simplify selection of transposition events and maximize their usefulness. Because Ds elements preferentially transpose to nearby genomic sites, they can be used in targeted mutagenesis of linked genes. To efficiently target all genes throughout the genome, we generated a large population of transgenic Arabidopsis plants containing the Ds launch pad construct, identified lines containing single Ds launch pad inserts, and mapped the positions of Ds launch pads in 89 lines. The integration sites of the Ds launch pads were relatively evenly distributed on all five chromosomes, except for a region of chromosomes 2 and 4 and the centromeric regions. This resource therefore provides access to the majority of the Arabidopsis genome for targeted tagging.
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1
Base de dados:
MEDLINE
Assunto principal:
Arabidopsis
/
Genoma de Planta
Idioma:
En
Revista:
Plant Physiol
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Reino Unido