Nucleolytic processing of a protein-bound DNA end by the E. coli SbcCD (MR) complex.
DNA Repair (Amst)
; 2(7): 795-807, 2003 Jul 16.
Article
em En
| MEDLINE
| ID: mdl-12826280
ABSTRACT
SbcCD and other Mre11/Rad50 (MR) complexes are implicated in the metabolism of DNA ends. They cleave ends sealed by hairpin structures and have been postulated to play roles in removing protein bound to DNA termini. Here we provide direct evidence that the Escherichia coli MR complex (SbcCD) removes protein from a protein-bound DNA end by inserting a double-strand break (DSB). These observations indicate a more complex biochemical action than has been assumed previously and argue that this class of protein has the potential to play a direct role in deprotecting protein-bound DNA ends in vivo.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Proteínas de Escherichia coli
/
Desoxirribonucleases
/
Proteínas de Ligação a DNA
/
Escherichia coli
/
Exodesoxirribonucleases
/
Exonucleases
Idioma:
En
Revista:
DNA Repair (Amst)
Assunto da revista:
BIOLOGIA MOLECULAR
/
BIOQUIMICA
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Reino Unido