[Long-term effects of 17 beta-estradiol in promoting 45Ca uptake and mineralized bone-like tissue formation in human osteoblast-like cell lines TE85].
Yao Xue Xue Bao
; 38(3): 181-4, 2003 Mar.
Article
em Zh
| MEDLINE
| ID: mdl-12830712
ABSTRACT
AIM:
To study the long-term effects of 17 beta-estradiol (E2) on cell proliferation, 45Ca up-take, and mineralized bone-like tissue formation in human osteoblast-like cell line TE85.METHODS:
Human osteoblast-like cell line TE85 was used as osteoblast cell model. Using methods of 3H-thymidine incorporation for cell proliferation and 45Ca deposit for calcium uptake, and Alizarin red S dye for mineralized bone-like tissue.RESULTS:
Compared with the cells of control group, 3H-thymidine incorporation into TE85 cells was significantly increased (85.65%, 93.42% and 106.58%, respectively) and 45Ca uptake was increased (101.35%, 130.9% and 169.5% respectively), after treated with E2 (0.1, 1.0, 10 nmol.L-1) for 14 days. The stained area of Alizarin red S in E2 treated cells was also increased obviously. ICI182,780, a specific antagonist of estrogen receptor, was shown to partly inhibit E2 induced actions with the inhibition ratio of 19.6% or 37.28% in both experiments of 3H-TdR and 45Ca uptake on the condition of E2 (1.0 nmol.L-1).CONCLUSION:
E2 was found to increase the mineralized bone-like tissue by enhancement of cell proliferation and promotion of calcium uptake, which were in favor of bone formation. These actions may be partly mediated by estrogen receptor.
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Base de dados:
MEDLINE
Assunto principal:
Osteoblastos
/
Radioisótopos de Cálcio
/
Estradiol
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
Zh
Revista:
Yao Xue Xue Bao
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
China