Beta-amyloid peptide-induced blood-brain barrier disruption facilitates T-cell entry into the rat brain.
Acta Histochem
; 105(2): 115-25, 2003.
Article
em En
| MEDLINE
| ID: mdl-12831163
ABSTRACT
Activated T-lymphocytes can migrate through the blood-brain barrier (BBB) and are able to invade the central nervous system (CNS). In the present study, we investigated whether disruption of the BBB leads to enhanced T-cell migration into the CNS. Amyloid-beta peptide 25-35 (A beta) or tumor necrosis factor-alpha (TNFalpha) were administered into the right common carotid artery of adult male Wistar rats. The agents were administered either alone, or were followed by a cell suspension of exogenously activated T-cells. Rats of other groups received activated or non-stimulated T-lymphocytes only. Sagittal brain sections were analyzed with immunohistochemistry of CD3 to reveal the presence of T-lymphocytes within the CNS parenchyma. Administration of activated T-cells alone led to T-cell migration into the brain. Infusion of either substances (A beta or TNFalpha) resulted in T-cell invasion of the CNS even when no exogenous T-cells were added. Infusion of either of the agents together with T-lymphocytes generated a more intense T-lymphocyte migration than in the other groups. Electron microscopic analysis and Evans-blue extravasation studies confirmed parallel disruption of the BBB. Our study demonstrates that A beta and TNFalpha induce enhanced T-lymphocyte migration towards the brain. This effect may be attributed at least partly to dysfunctioning of the BBB, but other mechanisms are also possible.
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Base de dados:
MEDLINE
Assunto principal:
Fragmentos de Peptídeos
/
Encéfalo
/
Barreira Hematoencefálica
/
Linfócitos T
/
Peptídeos beta-Amiloides
Limite:
Animals
Idioma:
En
Revista:
Acta Histochem
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Hungria