Collagen-induced activation of the M(r) 72,000 type IV collagenase in normal and malignant human fibroblastoid cells.
Cancer Res
; 52(16): 4540-4, 1992 Aug 15.
Article
em En
| MEDLINE
| ID: mdl-1322793
ABSTRACT
Although the M(r) 72,000 type IV collagenase (matrix metalloproteinase 2) has been implicated in a variety of normal and pathogenic processes, its activation mechanism in vivo is unclear. We have found that fibroblasts from normal and neoplastic human breast, as well as the sarcomatous human Hs578T and HT1080 cell lines, activate endogenous matrix metalloprotease 2 when cultured on type I collagen gels, but not on plastic, fibronectin, collagen IV, gelatin, matrigel, or basement membrane-like HR9 cell matrix. This activation is monitored by the zymographic detection of M(r) 59,000 and/or M(r) 62,000 species, requires 2-3 days of culture on vitrogen to manifest, is cycloheximide inhibitable, and correlates with an arborized morphology. A similar activation pattern was seen in these cells in response to Concanavalin A but not transforming growth factor beta or 12-O-tetradecanoylphorbol-13-acetate. The interstitial matrix may thus play an important role in regulating matrix degradation in vivo.
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Base de dados:
MEDLINE
Assunto principal:
Pele
/
Neoplasias da Mama
/
Carcinossarcoma
/
Colágeno
/
Colagenase Microbiana
/
Fibroblastos
Limite:
Humans
Idioma:
En
Revista:
Cancer Res
Ano de publicação:
1992
Tipo de documento:
Article