CDP-diacylglycerol synthesis in rat liver mitochondria.
FEBS Lett
; 312(2-3): 236-40, 1992 Nov 09.
Article
em En
| MEDLINE
| ID: mdl-1330695
ABSTRACT
CDP-diacylglycerol for polyglycerophosphatide biogenesis can be synthesized within rat liver mitochondria. This membrane-associated enzyme was predominantly located in the inner mitochondrial membrane. GTP had a significant effect in activating the microsomal CDP-diacylglycerol synthase, especially if the microsomes were preincubated with GTP in the presence of phosphatidic acid. This stimulatory effect of GTP on the microsomal enzyme was not detected in the mitochondrial fractions. The enzymes could be solubilized from the membrane fractions using CHAPS, and the detergent-soluble activity partially restored by addition of phospholipids. Mitochondrial and microsomal CDP-diacylglycerol synthase activity could be completely separated by anion-exchange column chromatography. The mitochondrial and microsomal CDP-diacylglycerol synthases appear to be two distinct enzymes with different localization and regulatory characteristics.
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Base de dados:
MEDLINE
Assunto principal:
Mitocôndrias Hepáticas
/
Diacilglicerol Colinofosfotransferase
/
Diglicerídeos de Citidina Difosfato
Limite:
Animals
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
Canadá