Direct activation of second messenger pathways mimics cell adhesion molecule-dependent neurite outgrowth.
J Cell Biol
; 118(3): 663-70, 1992 Aug.
Article
em En
| MEDLINE
| ID: mdl-1379246
ABSTRACT
We present evidence that direct activation of neuronal second messenger pathways in PC12 cells by opening voltage-dependent calcium channels mimics cell adhesion molecule (CAM)-induced differentiation of these cells. PC12 cells were cultured on monolayers of control 3T3 cells or 3T3 cells expressing transfected N-cadherin in the presence of KCl or a calcium channel agonist Bay K 8644. Both potassium depolarization and agonist-induced activation of calcium channels promoted substantial neurite outgrowth from PC12 cells cultured on control 3T3 monolayers and increased neurite outgrowth from those cultured on N-cadherin-expressing 3T3 monolayers. The potassium-induced response could be inhibited by L- and N-type calcium channel antagonists and by kinase inhibitor K-252b but was unaffected by pertussis toxin. In contrast activators of protein kinase C did not stimulate neurite outgrowth, and the neurite outgrowth response induced by activation of protein kinase A was not inhibited by calcium channel antagonists or pertussis toxin. These studies support the postulate that CAM-induced neuronal differentiation involves a specific transmembrane signaling pathway and suggest that activation of this pathway after CAM binding may be more important for the neurite outgrowth response than CAM-dependent adhesion per se.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Sistemas do Segundo Mensageiro
/
Moléculas de Adesão Celular
/
Neuritos
Limite:
Animals
Idioma:
En
Revista:
J Cell Biol
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
Reino Unido