Preparation and use of the poly-L-lysine-gold probe: a differential marker of glomerular anionic sites.
Histochemistry
; 98(1): 67-72, 1992 Aug.
Article
em En
| MEDLINE
| ID: mdl-1385368
ABSTRACT
The conditions required for the production of a polylysine-coated gold (PL-G) complex, which shows optimal sensitivity for the demonstration of tissue anionic sites, expressed under different conditions of pH have been investigated. Problems encountered with this complex have been compared with those found with other methods of conjugation of polylysine to colloidal gold. The performance of a bovine serum albumin (BSA)-stabilized PL-G complex was examined against other PL-G conjugates, including complexes that are commercially available, for the detection of heterogeneous glomerular anionic site populations, expressed at pH 2.5 and pH 7.0.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Polilisina
/
Ouro
/
Glomérulos Renais
Idioma:
En
Revista:
Histochemistry
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
Reino Unido