Simultaneous determination of cotinine and trans-3'-hydroxycotinine in human serum by high-performance liquid chromatography.
J Chromatogr
; 577(2): 358-61, 1992 Jun 10.
Article
em En
| MEDLINE
| ID: mdl-1400767
ABSTRACT
A high-performance liquid chromatographic method with ultraviolet photometric detection has been developed for the quantitation of cotinine and trans-3'-hydroxycotinine in human serum. A solid-phase extraction procedure was performed for the analytes and the internal standard, N-ethylnorcotinine, before chromatography. The use of a 30-cm reversed-phase column and a mobile phase of water-methanol-0.1 M sodium acetate-acetonitrile (6724.56.52, v/v), pH 4.3, prevented the co-elution of caffeine with cotinine. The limit of quantitation observed with this method was 5 ng/ml for both cotinine and trans-3'-hydroxycotinine. The present method proved useful for the determination of serum levels of these metabolites, correlating with nicotine daily intake.
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Base de dados:
MEDLINE
Assunto principal:
Cotinina
Limite:
Humans
Idioma:
En
Revista:
J Chromatogr
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
Itália