Cellular and molecular studies on the early stages of human hematopoietic differentiation in culture of pure progenitors.
Leukemia
; 6 Suppl 4: 41-3, 1992 Nov.
Article
em En
| MEDLINE
| ID: mdl-1434830
ABSTRACT
Methodology has been developed that enables virtually complete purification and recovery of early hematopoietic progenitors from human adult blood, a minority of which is multipotent and endowed with self-renewal capacities, i.e., exhibits stem cell properties. This report briefly reviews (i) the key steps involved in the progenitor purification and assay procedure; (ii) the characterization of "pure" progenitors at the level of membrane antigen pattern and response to HGFs; (iii) the development of a liquid suspension culture for the pure progenitors, which allows synchronized and selective erythroid or GM differentiation, and hence may be utilized for the analysis of molecular mechanisms underlying early and late stages of hematopoiesis; (iv) the study of the expression and modulation of HGFRs expressed on progenitors.
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Base de dados:
MEDLINE
Assunto principal:
Células-Tronco Hematopoéticas
Limite:
Humans
Idioma:
En
Revista:
Leukemia
Assunto da revista:
HEMATOLOGIA
/
NEOPLASIAS
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
Itália