Phosducin-like proteins in Dictyostelium discoideum: implications for the phosducin family of proteins.
EMBO J
; 22(19): 5047-57, 2003 Oct 01.
Article
em En
| MEDLINE
| ID: mdl-14517243
ABSTRACT
Retinal phosducin is known to sequester transducin Gbetagamma, thereby modulating transducin activity. Phosducin is a member of a family of phosducin-like proteins (PhLP) found in eukaryotes. Phylogeny of 33 phosducin-like proteins from metazoa, plants and lower eukaryotes identified three distinct groups named phosducin-I-III. We discovered three phlp genes in Dictyostelium, each encoding a phosducin-like protein of a different group. Disruption of the phlp1 gene strongly impaired G-protein signalling, apparently due to mislocalization of Gbetagamma in phlp1-null cells. GFP-Gbeta and GFP-Ggamma are membrane associated in wild-type cells, but cytosolic in phlp1-null cells. Phlp2 disruption is lethal due to a synchronous collapse of the cells after 16-17 cell divisions. Phlp3 disruptants show no abnormal phenotype. These results establish a role for phosducin-like proteins in facilitating folding, localization or function of proteins, in addition to modulating G-protein signalling.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Transporte
/
Família Multigênica
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Dictyostelium
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Proteínas do Tecido Nervoso
Limite:
Animals
Idioma:
En
Revista:
EMBO J
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Holanda