A novel docking site on Mediator is critical for activation by VP16 in mammalian cells.
EMBO J
; 22(24): 6494-504, 2003 Dec 15.
Article
em En
| MEDLINE
| ID: mdl-14657022
ABSTRACT
ARC92/ACID1 was identified as a novel specific target of the herpes simplex transactivator VP16 using an affinity purification procedure. Characterization of the protein revealed tight interactions with human Mediator mediated through a von Willebrand type A domain. ARC92/ACID1 further contains a novel activator-interacting domain (ACID), which it shares with at least one other human gene termed PTOV1/ACID2. The structure of ARC92/ACID1 is of ancient origin but is conserved in mammals and in selected higher eukaryotes. A subpopulation of Mediator is associated with ARC92/ACID1, which is specifically required for VP16 activation both in vitro and in mammalian cells, but is dispensable for other activators such as SP1. Despite many known targets of VP16, ARC92/ACID1 appears to impose a critical control on transcription activation by VP16 in mammalian cells.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteína Vmw65 do Vírus do Herpes Simples
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
EMBO J
Ano de publicação:
2003
Tipo de documento:
Article
País de afiliação:
Alemanha