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Solution structure of the highly acidic protein HI1450 from Haemophilus influenzae, a putative double-stranded DNA mimic.
Parsons, Lisa M; Yeh, Deok Cheon; Orban, John.
Afiliação
  • Parsons LM; Center for Advanced Research in Biotechnology, University of Maryland Biotechnology Institute, Rockville, Maryland 20850, USA.
Proteins ; 54(3): 375-83, 2004 Feb 15.
Article em En | MEDLINE | ID: mdl-14747986
ABSTRACT
The solution structure of the acidic protein HI1450 from Haemophilus influenzae has been determined by NMR spectroscopy. HI1450 has homologues in ten other bacterial species including Escherichia coli, Vibrio cholerae, and Yersinia pestis but there are no functional assignments for any members of the family. Thirty-one of the amino acids in this 107-residue protein are aspartates or glutamates, contributing to an unusually low pI of 3.72. The secondary structure elements are arranged in an alpha-alpha-beta-beta-beta-beta order with the two alpha helices packed against the same side of an anti-parallel four-stranded beta meander. Two large loops, one between beta1 and beta2 and the other between beta2 and beta3 bend almost perpendicularly across the beta-strands in opposite directions on the non-helical side of the beta-sheet to form a conserved hydrophobic cavity. The HI1450 structure has some similarities to the structure of the double-stranded DNA (dsDNA) mimic uracil DNA glycosylase inhibitor (Ugi) including the distribution of surface charges and the position of the hydrophobic cavity. Based on these similarities, as well as having a comparable molecular surface to dsDNA, we propose that HI1450 may function as a dsDNA mimic in order to inhibit or regulate an as yet unidentified dsDNA binding protein.
Assuntos
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Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / DNA / Haemophilus influenzae / Mimetismo Molecular / Ressonância Magnética Nuclear Biomolecular Idioma: En Revista: Proteins Assunto da revista: BIOQUIMICA Ano de publicação: 2004 Tipo de documento: Article País de afiliação: Estados Unidos
Buscar no Google
Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / DNA / Haemophilus influenzae / Mimetismo Molecular / Ressonância Magnética Nuclear Biomolecular Idioma: En Revista: Proteins Assunto da revista: BIOQUIMICA Ano de publicação: 2004 Tipo de documento: Article País de afiliação: Estados Unidos