Biocompatible nanocrystalline octacalcium phosphate thin films obtained by pulsed laser deposition.
Biomaterials
; 25(13): 2539-45, 2004 Jun.
Article
em En
| MEDLINE
| ID: mdl-14751739
ABSTRACT
We extended for the first time pulsed laser ablation to the deposition of octacalcium phosphate Ca8H2(PO4)6.5H2O (OCP) thin films. The depositions were performed with a pulsed UV laser source (lambda=248 nm, tau> or =20 ns) in a flux of hot water vapors. The targets were sintered from crystalline OCP powder and the laser ablation fluence was set at values of 1.5-2 J/cm2. During depositions the collectors, Si or Ti substrates, were maintained at a constant temperature within the range 20-200 degrees C. The resulting structures were submitted to heat treatment in hot water vapors for up to 6 h. The best results were obtained at a substrate temperature of 150 degrees C during both deposition and post-deposition treatment. High-resolution electron microscopy and XRD at grazing incidence indicated that the coatings obtained were made of nanocrystalline OCP. Cross-section TEM investigations showed that the coatings contained droplets stacked on Ti substrates as well as distributed across the entire thickness of the arborescence-like structure layers. The results of WST-1 assay, cell adherence, DNA replication, and caspase-1 activity confirmed the good biocompatibility of the coatings.
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Base de dados:
MEDLINE
Assunto principal:
Materiais Biocompatíveis
/
Fosfatos de Cálcio
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Biomaterials
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Romênia