Structural analysis of saponins from medicinal herbs using electrospray ionization tandem mass spectrometry.
J Am Soc Mass Spectrom
; 15(2): 133-41, 2004 Feb.
Article
em En
| MEDLINE
| ID: mdl-14766280
ABSTRACT
The underivatized saponins from Tribulus terrestris and Panax ginseng have been investigated by electrospray ionization multi-stage tandem mass spectrometry (ESI-MS(n)). In ESI-MS spectra, a predominant [M + Na](+) ion in positive mode and [M - H](-) ion in negative mode were observed for molecular mass information. Multi-stage tandem mass spectrometry of the molecular ions was used for detailed structural analysis. Fragment ions from glycoside cleavage can provide information on the mass of aglycone and the primary sequence and branching of oligosaccharide chains in terms of classes of monosaccharides. Fragment ions from cross-ring cleavages of sugar residues can give some information about the linkages between sugar residues. It was found that different alkali metal-cationized adducts with saponins have different degrees of fragmentation, which may originate from the different affinity of a saponin with each alkali metal in the gas phase. ESI-MS(n) has been proven to be an effective tool for rapid determination of native saponins in extract mixtures, thus avoiding tedious derivatization and separation steps.
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Base de dados:
MEDLINE
Assunto principal:
Plantas Medicinais
/
Saponinas
/
Medicamentos de Ervas Chinesas
/
Espectrometria de Massas por Ionização por Electrospray
/
Tribulus
/
Panax
Idioma:
En
Revista:
J Am Soc Mass Spectrom
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
China