Cyanovirin-N inhibits AIDS virus infections in vaginal transmission models.
AIDS Res Hum Retroviruses
; 20(1): 11-8, 2004 Jan.
Article
em En
| MEDLINE
| ID: mdl-15000694
ABSTRACT
The cyanobacterial protein cyanovirin-N (CV-N) potently inactivates diverse strains of HIV-1 and other lentiviruses due to irreversible binding of CV-N to the viral envelope glycoprotein gp120. In this study, we show that recombinant CV-N effectively blocks HIV-1(Ba-L) infection of human ectocervical explants. Furthermore, we demonstrate the in vivo efficacy of CV-N gel in a vaginal challenge model by exposing CV-N-treated female macaques (Macaca fascicularis) to a pathogenic chimeric SIV/HIV-1 virus, SHIV89.6P. All of the placebo-treated and untreated control macaques (8 of 8) became infected. In contrast, 15 of 18 CV-N-treated macaques showed no evidence of SHIV infection. Further, CV-N produced no cytotoxic or clinical adverse effects in either the in vitro or in vivo model systems. Together these studies suggest that CV-N is a good candidate for testing in humans as an anti-HIV topical microbicide.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas de Bactérias
/
Vagina
/
Proteínas de Transporte
/
Infecções por HIV
/
Síndrome de Imunodeficiência Adquirida dos Símios
/
Fármacos Anti-HIV
Tipo de estudo:
Clinical_trials
Limite:
Animals
/
Female
/
Humans
Idioma:
En
Revista:
AIDS Res Hum Retroviruses
Assunto da revista:
SINDROME DA IMUNODEFICIENCIA ADQUIRIDA (AIDS)
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Estados Unidos