Identification of differentially expressed genes in colorectal adenoma compared to normal tissue by suppression subtractive hybridization.
Int J Oncol
; 24(4): 987-94, 2004 Apr.
Article
em En
| MEDLINE
| ID: mdl-15010839
ABSTRACT
Altered gene expression that results in unrestricted growth and loss of differentiation is a hallmark of neoplastic cells. The goal of the present study was to determine early changes in gene expression profiles during colorectal carcinogenesis with emphasis on those genes whose expression is upregulated in adenoma tissue compared to adjacent normal colon. Using the suppression subtractive hybridization (SSH) approach on enriched colonic crypts we identified 14 candidate genes showing increased expression levels in the original adenoma sample. Subsequent dot blot, Northern blot, standard and real-time RT-PCR analysis confirmed increased transcript levels of ten genes in various subsets of colorectal adenoma and cancer samples. SERPINB5 and a yet uncharacterized HERV-H sequence showed the highest expression in adenoma and tumor samples without significant expression in the corresponding normal tissue. These genes might represent promising new candidates for colorectal cancer early detection.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Colorretais
/
Adenoma
/
Marcadores Genéticos
/
Regulação Neoplásica da Expressão Gênica
/
Colo
Tipo de estudo:
Diagnostic_studies
/
Screening_studies
Limite:
Humans
Idioma:
En
Revista:
Int J Oncol
Assunto da revista:
NEOPLASIAS
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Alemanha