Identification and expression of a Burkholderia pseudomallei collagenase in Escherichia coli.
Curr Microbiol
; 48(4): 300-4, 2004 Apr.
Article
em En
| MEDLINE
| ID: mdl-15057457
ABSTRACT
Extracellular protein profiles were compared for broth-grown cultures of Burkholderia pseudomallei and its avirulent close relative Burkholderia thailandensis. A number of protein bands were present in the B. pseudomallei profile but absent or less abundant in the B. thailandensis profile. Four such prominent secreted proteins were identified by using N-terminal sequencing coupled to searches of the B. pseudomallei genome sequence database. The genes for two proteins with similarity to carbohydrate-binding proteins, and a further protein homologous to known bacterial collagenases, were present in both B. pseudomallei and B. thailandensis. The putative collagenase gene was cloned and expressed as a fusion protein in Escherichia coli. Cell lysates from Escherichia coli containing the recombinant protein exhibited detectable gelatinase and collagenase activities.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas Recombinantes
/
Burkholderia pseudomallei
/
Colagenases
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Curr Microbiol
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
Reino Unido