[The effect of polyporus umbellatus polysaccharide on the immunosuppression property of culture supernatant of S180 cells].
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
; 20(2): 234-7, 2004 Mar.
Article
em Zh
| MEDLINE
| ID: mdl-15191734
AIM: To investigate the effect of polyporus umbellatus polysaccharide (PUPS) on the immunosuppression property of tumor cell line S180 culture supernatant. METHODS: The inhibitory effects of the culture supernatant of S180 cells in the presence or absence of PUPS on the ConA-induced mouse splenocyte proliferation, IL-2 production, killer activity and the reactivity of CTLL-2 cells to IL-2 were detected by MTT colorimetry. The effect of the culture supernatant on the IL-2Ralpha expression on murine splenocytes was detected by flow cytometry. RESULTS: The culture supernatant of S180 cells could strongly inhibit immunity in terms of the above five indexes, while PUPS could reverse the immunosuppression. CONCLUSION: PUPS can offset the immunosuppression of the supernatant from S180 cell culture, which may be mediated by down-regulating the synthesis and/or secretion of immunosuppressive substance by S180 cells.
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Base de dados:
MEDLINE
Assunto principal:
Polyporaceae
/
Polissacarídeos
/
Sarcoma 180
/
Meios de Cultivo Condicionados
Limite:
Animals
Idioma:
Zh
Revista:
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
Assunto da revista:
ALERGIA E IMUNOLOGIA
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
China