Detection of circulating Leishmania chagasi DNA for the non-invasive diagnosis of human infection.
Trans R Soc Trop Med Hyg
; 97(4): 391-5, 2003.
Article
em En
| MEDLINE
| ID: mdl-15259464
ABSTRACT
A polymerase chain reaction (PCR) assay for the detection of Leishmania spp. DNA in peripheral blood was optimized and evaluated for the diagnosis of human visceral leishmaniasis (VL) in Brazil during May 2001 to December 2002. Optimization of the technique resulted in a detection limit of 1.65 fg of purified L. (L.) chagasi DNA, equivalent to 1.65 x 10(-2) parasites. Leishmania DNA was detected in the blood of 48 of 53 patients with parasitologically-confirmed VL, which corresponds to a sensitivity of 91%. No DNA was detected in the peripheral blood of 15 healthy, non-exposed volunteers, giving a specificity of 100%. We conclude that detection of parasite DNA in peripheral blood offers a non-invasive, sensitive and rapid method for the detection of VL caused by L. (L.) chagasi.
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Base de dados:
MEDLINE
Assunto principal:
Reação em Cadeia da Polimerase
/
DNA de Protozoário
/
Leishmania
/
Leishmaniose Visceral
Tipo de estudo:
Diagnostic_studies
/
Evaluation_studies
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Trans R Soc Trop Med Hyg
Ano de publicação:
2003
Tipo de documento:
Article