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Meiotic initiation by the mos protein in Xenopus.
Yew, N; Mellini, M L; Vande Woude, G F.
Afiliação
  • Yew N; ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, Maryland 21702-1201.
Nature ; 355(6361): 649-52, 1992 Feb 13.
Article em En | MEDLINE | ID: mdl-1531698
When fully grown Xenopus oocytes are stimulated by progesterone, a period of protein synthesis is necessary for maturation. Synthesis of the mos proto-oncogene product, pp39mos, is necessary for the activation of M-phase promoting factor (MPF) in meiosis I. On the basis that mos is translated de novo on hormonal stimulation of Xenopus oocytes and that injecting mos RNA into oocytes induces their maturation, we have proposed that the mos protein is a candidate initiator of oocyte maturation, needed to trigger the conversion of precursor MPF into its active form. To determine whether mos is the only protein required for initiating maturation, we have produced a soluble, active recombinant mos protein and injected it into Xenopus oocytes. We report here that in the absence of protein synthesis that mos protein efficiently induces germinal vesicle breakdown and the activation of MPF. The oocytes, however, do not proceed into meiosis II. Thus, the mos protein fulfills the requirements of an initiator protein, but the synthesis of one or more additional proteins may be necessary to complete oocyte maturation.
Assuntos
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Base de dados: MEDLINE Assunto principal: Proteínas Proto-Oncogênicas / Meiose Limite: Animals / Female / Humans / Newborn Idioma: En Revista: Nature Ano de publicação: 1992 Tipo de documento: Article
Buscar no Google
Base de dados: MEDLINE Assunto principal: Proteínas Proto-Oncogênicas / Meiose Limite: Animals / Female / Humans / Newborn Idioma: En Revista: Nature Ano de publicação: 1992 Tipo de documento: Article