The multidrug resistance and apoptosis evaluation in acute myeloid leukemia cells after the in vitro doxorubicin treatment.
Acta Medica (Hradec Kralove)
; 47(3): 181-5, 2004.
Article
em En
| MEDLINE
| ID: mdl-15568736
ABSTRACT
The apoptosis failure in cytostatic treatment of haemoblastosis is one of the means of chemoresistance. We were interested in the relationship of the after-doxorubicin-treatment-AML cells apoptosis and the immunophenotype, selected clinical and laboratory parameters, and also the P-gp, MRP, LRP, Bcl-2, Bax proteins expression. All analysis were evaluated with the flow cytometry method. To detect apoptotic cells in the sample, we used three methods:
annexin test, TUNEL (TdT-mediated dUTP nick end labeling) test, and caspase 8 detection. After the cell cultivation the statisticaly important increase of apoptotic cells in the culture was apparent. The relation between the AML blast in vitro reaction and some clinical parameters such as the age of patient, white blood cell count, and blast percentage was also observed.
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Base de dados:
MEDLINE
Assunto principal:
Doxorrubicina
/
Leucemia Mieloide
/
Apoptose
/
Proteínas Associadas à Resistência a Múltiplos Medicamentos
/
Antibióticos Antineoplásicos
Limite:
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Acta Medica (Hradec Kralove)
Assunto da revista:
MEDICINA
Ano de publicação:
2004
Tipo de documento:
Article