Membrane protein crystallization in amphiphile phases: practical and theoretical considerations.
Prog Biophys Mol Biol
; 88(3): 339-57, 2005 Jul.
Article
em En
| MEDLINE
| ID: mdl-15652249
ABSTRACT
Integral membrane proteins are amphiphilic molecules. In order to enable chromatographic purification and crystallization, a complementary amphiphilic microenvironment must be created and maintained. Various types of amphiphilic phases have been employed in crystallizations and intricate amphiphilic microenvironmental structures have resulted from these and are found inside membrane protein crystals. In this review the process of crystallization is put into the context of amphiphile phase transitions. Finally, practical factors are considered and a pragmatic way is suggested to pursue membrane protein crystallization trials.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas
/
Modelos Moleculares
/
Cristalização
/
Proteínas de Membrana
/
Modelos Químicos
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Prog Biophys Mol Biol
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Estados Unidos