Asymmetric reduction of ethyl 4-chloro-3-oxobutanoate to ethyl (R)-4-chloro-3-hydroxybutanoate with two co-existing, recombinant Escherichia coli strains.
Biotechnol Lett
; 27(2): 119-25, 2005 Jan.
Article
em En
| MEDLINE
| ID: mdl-15703875
ABSTRACT
Two recombinant strains, E. coli M15 (pQE30-alr0307) and E. coli M15 (pQE30-gdh0310), which were constructed to express, respectively, an NADPH-dependent aldehyde reductase gene and a glucose dehydrogenase gene, were mixed in an appropriate ratio and used for the asymmetric reduction of ethyl 4-chloro-3-oxobutanoate to ethyl (R)-4-chloro-3-hydroxybutanoate. The former strain acted as catalyst and the latter functioned in NADPH regeneration. The biotransformation was completed effectively without any addition of glucose dehydrogenase or NADP+/NADPH. An optical purity of 99% (ee) was obtained and the product yield reached 90.5% from 28.5 mM substrate.
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Base de dados:
MEDLINE
Assunto principal:
Microbiologia Industrial
/
Butiratos
/
Escherichia coli
/
Acetoacetatos
Idioma:
En
Revista:
Biotechnol Lett
Ano de publicação:
2005
Tipo de documento:
Article