[Determination of deoxyribonuceic acids by resonance light scattering technique with benzidine in the presence of the sensitizing sodium dodecyl sulfate].
Guang Pu Xue Yu Guang Pu Fen Xi
; 24(9): 1035-8, 2004 Sep.
Article
em Zh
| MEDLINE
| ID: mdl-15762516
ABSTRACT
The resonance light scattering (RLS) spectra of benzidine with deoxyribonucleic acid (ctDNA) in the presence of Sodium Dodecyl Sulfate had been studied. RLS intensity of SDS-benzidine system was enhanced by trace ctDNA and was proportional to the concentrationof ctDNA. Under optima conditions, the linear ranges of the calibration were 0.3-4 mg x L(-1), the linear regression equation was I = 37.17c (ctDNA, mg x L(-1)) + 208.9, r = 0.9992. The limit of detection were 0.17 mg x L(-1) (3delta), RSD% = 3.5%. The method had been applied to the determination of DNA in synthetic samples and gene tobacco sample, and the results were satisfactory.
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Base de dados:
MEDLINE
Assunto principal:
Espalhamento de Radiação
/
Dodecilsulfato de Sódio
/
Espectrometria de Fluorescência
/
Benzidinas
/
DNA
Idioma:
Zh
Revista:
Guang Pu Xue Yu Guang Pu Fen Xi
Ano de publicação:
2004
Tipo de documento:
Article
País de afiliação:
China