Sandwich ELISA detection of Clostridium perfringens cells and alpha-toxin from field cases of necrotic enteritis of poultry.
Vet Microbiol
; 106(3-4): 259-64, 2005 Apr 10.
Article
em En
| MEDLINE
| ID: mdl-15778032
ABSTRACT
Sandwich ELISAs (sELISAs) for the detection of Clostridium perfringens cells and alpha-toxin were developed and used to screen intestinal samples from normal broiler chickens and from clinical cases of necrotic enteritis. The assays clearly distinguished between the two sets of samples. The sELISA absorbance values from samples obtained from the majority of healthy birds were low and those from the majority of necrotic enteritis cases were high. Together, the assays provide a suitable test for the rapid screening for the diagnosis of necrotic enteritis in poultry.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Fosfolipases Tipo C
/
Doenças das Aves Domésticas
/
Ensaio de Imunoadsorção Enzimática
/
Galinhas
/
Infecções por Clostridium
/
Clostridium perfringens
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Vet Microbiol
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Reino Unido