Functional reassembly of the Escherichia coli maltose transporter following purification of a MalF-MalG subassembly.
J Bacteriol
; 187(8): 2908-11, 2005 Apr.
Article
em En
| MEDLINE
| ID: mdl-15805537
ABSTRACT
Taking advantage of a chaperone-like function of MalK, a stable complex of MalF-MalG could be solubilized from the Escherichia coli membrane and purified in high yield in the absence of MalK. This MalF-MalG complex was competent for efficient reassembly of a functional MalFGK(2) maltose transporter complex both in detergent solution and in proteoliposomes.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Transporte de Monossacarídeos
/
Transportadores de Cassetes de Ligação de ATP
/
Proteínas de Escherichia coli
/
Escherichia coli
Idioma:
En
Revista:
J Bacteriol
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Estados Unidos