The BeWo choriocarcinoma cell line as a model of iodide transport by placenta.
Placenta
; 26(5): 380-6, 2005 May.
Article
em En
| MEDLINE
| ID: mdl-15850642
ABSTRACT
Cultured human choriocarcinoma cells of the BeWo line exhibited saturable accumulation of radioiodide. Inhibition by competing anions followed the affinity series perchlorate >> iodide > or = thiocyanate, consistent with uptake through the thyroid iodide transporter, NIS, whose messenger RNA was found in BeWo cells, and whose protein was distributed towards the apical pole of the cells. Efflux obeyed first order kinetics and was inhibited by DIDS, an antagonist of anion exchangers including pendrin, whose messenger RNA was also present. In cultures where iodide uptake through NIS was blocked with excess perchlorate, radioiodide accumulation was stimulated by exposure to medium in which physiological anions were replaced by 2-morpholinoethanesulfonic acid (MES), consistent with the operation of an anion exchange mechanism taking up iodide. Chloride in the medium was more effective than sulfate at inhibiting this uptake, matching the ionic specificity of pendrin. These studies provide evidence that the trophoblast accumulates iodide through NIS and releases it to the fetal compartment through pendrin.
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Base de dados:
MEDLINE
Assunto principal:
Placenta
/
Neoplasias Uterinas
/
Coriocarcinoma
/
Iodetos
Limite:
Female
/
Humans
Idioma:
En
Revista:
Placenta
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Austrália