Gene expression profiling in hypothalamus of immobilization-stressed mouse using cDNA microarray.
Brain Res Mol Brain Res
; 135(1-2): 293-300, 2005 Apr 27.
Article
em En
| MEDLINE
| ID: mdl-15857693
ABSTRACT
To investigate the effects of repeated immobilization-stress challenge on HPA axis, genomic transcriptome in the hypothalamus of immobilization-stressed mouse was analyzed by using cDNA microarray. With the 1.5-fold cutoff of arbitrary criteria, the expression levels of 108 genes out of 6016 genes were significantly modulated in the hypothalamus by the stress. Energy metabolism-, lipid metabolism-, and apoptosis- and signal transduction-related genes were activated while DNA repair-, protein biosynthesis-, and structure integrity-related genes were down-regulated in the hypothalamus. Eighteen genes among them were selected for RT-PCR analysis to confirm the change of their expression levels on agarose gels. Besides, dozens of novel genes, which have not been previously reported, were screened to be modulated by the immobilization stress through the transcriptome analysis. These genes are related to apoptosis, tumor-suppression, DNA-binding and protein folding, and thus may be used as potential targets for the development of therapeutics of chronic stress or depressant.
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Base de dados:
MEDLINE
Assunto principal:
Restrição Física
/
Regulação da Expressão Gênica
/
Análise de Sequência com Séries de Oligonucleotídeos
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Perfilação da Expressão Gênica
/
Hipotálamo
Limite:
Animals
Idioma:
En
Revista:
Brain Res Mol Brain Res
Assunto da revista:
BIOLOGIA MOLECULAR
/
CEREBRO
Ano de publicação:
2005
Tipo de documento:
Article