A selective and rapid method for the quantification of captopril in human plasma using liquid chromatography/selected reaction monitoring mass spectrometry.
J Pharm Biomed Anal
; 37(5): 1073-80, 2005 Apr 29.
Article
em En
| MEDLINE
| ID: mdl-15862687
ABSTRACT
A specific hyphenated high performance liquid chromatography-mass spectrometric (LC-MS/MS) assay was developed for the determination of captopril in plasma. The drug was extracted from plasma using liquid-liquid extraction with a mixture of diethyletherdichloromethane. After the addition of the internal standard, samples were applied to a prepacked C8 Waters Symmetry column. The ion trap MS/MS detector was equipped with electrospray ionization (ESI) source operating in the positive ion mode. Drug determination was accomplished monitoring captopril at molecular ion m/z 218 and MS/MS (daughter) at m/z 171.6. The method was applied to captopril determination in human plasma after the administration of captopril 50 mg tablets to healthy volunteers who have participated in a pharmacokinetic study. The method was proved to be specific and precise by testing six different plasma batches. Linearity was established for the range of concentrations 25-3000 ng/ml with a regression factor of 0.9995. Intra-day accuracy ranged from 90.16 to 96.18%, while the intra-day precision ranged from 2.60 to 9.66% at the concentrations of 75, 1440 and 2500 ng/ml. Inter-day precision of the method ranged from 5.04 to 10.10%. This validated method of analysis was successfully applied to human plasma analyses after the administration of a single dose of 50 mg captopril tablets to healthy volunteers.
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Base de dados:
MEDLINE
Assunto principal:
Captopril
Tipo de estudo:
Diagnostic_studies
Limite:
Humans
/
Male
Idioma:
En
Revista:
J Pharm Biomed Anal
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Jordânia