In vivo DNA gene electro-transfer: a systematic analysis of different electrical parameters.
J Gene Med
; 7(11): 1475-81, 2005 Nov.
Article
em En
| MEDLINE
| ID: mdl-15991255
ABSTRACT
BACKGROUND:
Intramuscular plasmid injection followed by electroporation is an efficient method for gene therapy or vaccination. Several protocols have been described that give good transduction levels with several reporter genes.METHODS:
In this work we have explored the efficiency of gene delivery upon variation of the different electrical parameters such as pulse length frequency and voltage monitoring both on short- and long-term protein production.RESULTS:
Having defined the best performing parameters, we have designed a short electric treatment that gives good levels of plasmid-encoded protein in different species such as mice, rabbits and monkeys.
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Base de dados:
MEDLINE
Assunto principal:
DNA
/
Técnicas de Transferência de Genes
/
Eletroporação
Tipo de estudo:
Guideline
Limite:
Animals
Idioma:
En
Revista:
J Gene Med
Assunto da revista:
BIOLOGIA MOLECULAR
/
GENETICA MEDICA
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Itália