Myostatin gene silenced by RNAi show a zebrafish giant phenotype.
J Biotechnol
; 119(4): 324-31, 2005 Oct 10.
Article
em En
| MEDLINE
| ID: mdl-16005092
ABSTRACT
Myostatin is a member of the transforming growth factor-beta (TGF-beta) family that functions as a negative regulator of skeletal muscle development and growth. Recently, it has been reported that the transgenic zebrafish expressing myostatin prodomain exhibited an increased number of fiber in skeletal muscle. Other novel results suggest that myostatin plays a mayor role during myogenesis, apart from inhibition of proliferation as well as differentiation. We have investigated the ability of double-stranded RNA (dsRNA) to inhibit myostatin function in the zebrafish. By microinjection dsRNA, corresponding to biologically active C-terminal domain from aminoacid 268 to end codon of tilapia myostatin protein, we produced an increased body mass in treated fish. The dsRNA injection in early development stage in zebrafish produced hyperplasia or hypertrophy. In addition, the interference of gene function showed a strong dependence on the amount of dsRNA.
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Base de dados:
MEDLINE
Assunto principal:
Peixe-Zebra
/
Fator de Crescimento Transformador beta
/
Músculo Esquelético
/
Interferência de RNA
Limite:
Animals
Idioma:
En
Revista:
J Biotechnol
Assunto da revista:
BIOTECNOLOGIA
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Cuba