Selective inhibition of divergent seryl-tRNA synthetases by serine analogues.
FEBS Lett
; 579(20): 4344-8, 2005 Aug 15.
Article
em En
| MEDLINE
| ID: mdl-16054140
ABSTRACT
Seryl-tRNA synthetases (SerRSs) fall into two distinct evolutionary groups of enzymes, bacterial and methanogenic. These two types of SerRSs display only minimal sequence similarity, primarily within the class II conserved motifs, and possess distinct modes of tRNA(Ser) recognition. In order to determine whether the two types of SerRSs also differ in their recognition of the serine substrate, we compared the sensitivity of the representative methanogenic and bacterial-type SerRSs to serine hydroxamate and two previously unidentified inhibitors, serinamide and serine methyl ester. Our kinetic data showed selective inhibition of the methanogenic SerRS by serinamide, suggesting a lack of mechanistic uniformity in serine recognition between the evolutionarily distinct SerRSs.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Serina
/
Serina-tRNA Ligase
/
Inibidores Enzimáticos
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Estados Unidos