Study of the interaction of hexa-amine cobalt (III) ion with DNA by a resonance light scattering technique and its analytical application.

ABSTRACT

The effect of hexa-amine cobalt cations on the DNA condensation in aqueous solution was investigated by resonance light scattering (RLS). When the relative concentration of hexa-amine cobalt (III) cations to DNA is in the appropriate range, the cations will induce DNA condensation and aggregation, which results in a strong RLS spectrum characterized by a peak at 290.0 nm. The RLS technique is a powerful tool for monitoring DNA condensation and, under optimal conditions, the enhanced RLS intensity at 290.0 nm was proportional to the concentration of DNA in the range 0.01-6.0 microg/mL. Based on this, a sensitive and convenient analysis method for the microdetermination of DNA was established. The detection limit (3 s) of calf thymus DNA by the proposed method is 1.9 ng/mL and few substances interfere in the DNA determination.