Evidence for proprotein convertase activity in the endoplasmic reticulum/early Golgi.
FEBS Lett
; 579(25): 5621-5, 2005 Oct 24.
Article
em En
| MEDLINE
| ID: mdl-16213495
ABSTRACT
Processing of precursor proteins by the proprotein convertases is thought to occur mainly in the trans-Golgi network or post-Golgi compartments. Such cleavage is inhibited by the prosegment of the convertases. During our studies of the use of the inhibitory prosegment of PC1, we noticed that a construct containing the prosegment fused to the C-terminal secretory granule sorting domain was cleaved in the endoplasmic reticulum (ER) at a pair of basic residues, best recognized by furin and PC7. This was further confirmed when this construct was fused at the C-terminus with a KDEL ER-retention signal. This suggests that the convertases could cleave some substrates within the ER, possibly by displacing the inhibitory prosegment associated with them.
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Base de dados:
MEDLINE
Assunto principal:
Rede trans-Golgi
/
Pró-Proteína Convertase 1
/
Retículo Endoplasmático
Limite:
Animals
/
Humans
Idioma:
En
Revista:
FEBS Lett
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Canadá