Spectral- and frequency-encoded fluorescence imaging.
Opt Lett
; 30(20): 2760-2, 2005 Oct 15.
Article
em En
| MEDLINE
| ID: mdl-16252766
ABSTRACT
A method for obtaining fluorescence images with a high number of resolvable points by using spectral and frequency encoding is presented. Broadband excitation light is encoded with a wavelength-dependent frequency modulation and dispersed onto the sample with a grating to simultaneously illuminate an entire image line. The Fourier transform of the frequency-encoded fluorescence emission provides one line of the image. Mechanical scanning along a direction orthogonal to the wavelength-encoded axis allows creation of the two-dimensional fluorescent image. This method is applicable for developing submillimeter diameter endoscopes. The principles of the technique are validated by imaging indocyanine green fluorescence in microfluidic channels.
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Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Fluorescência
/
Aumento da Imagem
/
Microscopia Confocal
/
Endoscopia
/
Microscopia de Fluorescência
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Opt Lett
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Estados Unidos