Establishment and characterization of a malignant melanocytic tumor cell line expressing the ret oncogene.
Oncogene
; 7(8): 1491-6, 1992 Aug.
Article
em En
| MEDLINE
| ID: mdl-1630812
ABSTRACT
We established a cell line (designated Mel-ret) from a melanocytic tumor developed in a metallothionein/ret transgenic mouse. Unlike primary melanocytic tumors, which did not show malignant features, when the Mel-ret cells were transplanted into nude mice they invaded into surrounding tissues and had metastatic ability. Although the Ret proteins were expressed at similar levels in the cell line and the primary tumors, the level of tyrosine phosphorylation in the Mel-ret cells was much higher than that in the primary tumors. In particular, an 85-kDa tyrosine-phosphorylated band was specifically detected in the Mel-ret cells. These results suggest that the increase in tyrosine phosphorylation may be responsible for malignant transformation of the Mel-ret cells. Immunofluorescence and cell fractionation studies showed that the Ret proteins and most of tyrosine-phosphorylated proteins in the Mel-ret cells localized in the membrane fraction. No activation of phosphatidyl-inositol-3 kinase (PI-3 kinase), a target protein for several tyrosine kinases, was detected in the Mel-ret cells.
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Base de dados:
MEDLINE
Assunto principal:
Oncogenes
/
Melanoma Experimental
/
Proteínas Tirosina Quinases
/
Transformação Celular Neoplásica
/
Proteínas Proto-Oncogênicas
/
Receptores Proteína Tirosina Quinases
/
Proteínas de Drosophila
Limite:
Animals
Idioma:
En
Revista:
Oncogene
Assunto da revista:
BIOLOGIA MOLECULAR
/
NEOPLASIAS
Ano de publicação:
1992
Tipo de documento:
Article
País de afiliação:
Japão