Ultrasound-mediated gene transfer into neuronal cells.
J Biotechnol
; 122(4): 393-411, 2006 Apr 20.
Article
em En
| MEDLINE
| ID: mdl-16309774
ABSTRACT
A new field of gene transfer is emerging as a simple, effective means to drive the expression foreign genes in cells ultrasound-mediated gene transfer or sonoporation. We report here that sonoporation is an effective means of gene transfer for cultured neurons, a cell type that has been difficult to transfect. Neuronal cell types that are effectively sonoporated include chick retinal neurons, chick dorsal forebrain, chick optic tectum, PC12 cells, rat cerebellar neurons and mouse hippocampal neurons. Depending on the type of cell and conditions of sonoporation the transfection efficacy was as high as 20%. Sonoporation of plasmid DNA was effective for cells adherent to a substrate and for free-floating cells that were freshly dissociated. In the free-floating preparations, between 60 and 95% of the cells that were transfected were neuronal, as much as 90% higher than that observed for other methods of gene transfer including adenovirus and lipid-based transfection methods. We conclude that sonoporation is a simple, effective and inexpensive means by which to preferentially transfect DNA into neuronal cells.
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Base de dados:
MEDLINE
Assunto principal:
Ultrassom
/
Transfecção
/
Técnicas de Transferência de Genes
/
Eletroporação
/
Neurônios
Limite:
Animals
Idioma:
En
Revista:
J Biotechnol
Assunto da revista:
BIOTECNOLOGIA
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Estados Unidos