HIV-1 Tat neurotoxicity in primary cultures of rat midbrain fetal neurons: changes in dopamine transporter binding and immunoreactivity.
Neurosci Lett
; 395(3): 235-9, 2006 Mar 13.
Article
em En
| MEDLINE
| ID: mdl-16356633
ABSTRACT
HIV-1 neurotoxic proteins (Tat, gp120) are believed to play a major role in pathogenesis of dementia in a significant portion of the AIDS patient population. Dopaminergic systems appear to be particularly important in HIV-associated dementia. In the current studies, we determined that primary cell cultures prepared from the midbrain of 18-day-old rat fetuses are sensitive to Tat neurotoxicity and investigated the possible effects of Tat on DAT-specific ligand binding and DAT immunoreactivity in rat fetal midbrain cultures. We found that Tat neurotoxicity was associated with a significant decrease in [3H]WIN 35428 binding. Immunostaining of cell cultures with antibodies recognizing the C-end epitope of DAT did not reveal significant changes in DAT immunoreactivity. The results of this study implicate involvement of monoamine transmission systems in HIV-associated dementia.
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Base de dados:
MEDLINE
Assunto principal:
Mesencéfalo
/
Produtos do Gene tat
/
HIV-1
/
Proteínas da Membrana Plasmática de Transporte de Dopamina
/
Neurônios
/
Neurotoxinas
Limite:
Animals
/
Pregnancy
Idioma:
En
Revista:
Neurosci Lett
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Estados Unidos