In vitro isolation and characterisation of the first canine Neospora caninum isolate in Australia.
Vet Parasitol
; 137(3-4): 355-63, 2006 Apr 30.
Article
em En
| MEDLINE
| ID: mdl-16487658
ABSTRACT
Neospora caninum was isolated and established in vitro from the skin lesion of a naturally infected dog. The identity of the parasite was evaluated by immunofluorescent antibody test (IFAT), microscopy, Western blotting and polymerase chain reaction (PCR). N. caninum DNA was detected in the whole blood, serum, skin lesion, rectal scrapings and faeces of the infected dog utilising a nested PCR targeting the Nc-5 gene of N. caninum. Antigenic and genetic characterisation of the isolate, designated WA-K9, at a number of loci including the Nc-5 gene, heat shock protein 70 (HSP-70) gene, alpha-tubulin and beta-tubulin genes revealed no variation between this isolate and two N. caninum isolates from different geographic areas. Clinical aspects of this case, which included cutaneous and neurological disease, are also discussed.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
DNA de Protozoário
/
Coccidiose
/
Neospora
/
Doenças do Cão
Limite:
Animals
País/Região como assunto:
Oceania
Idioma:
En
Revista:
Vet Parasitol
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Austrália