Expression of recombinant Streptolysin O and specific antibody production.
J Mol Microbiol Biotechnol
; 10(1): 64-8, 2005.
Article
em En
| MEDLINE
| ID: mdl-16491027
ABSTRACT
Streptolysin O (SLO), an oxygen-labile cytolysin, is the cholesterol-binding exotoxin of hemolytic streptococci. Besides microbiological and pathological interests, this cytolysin has been used as a tool for permeabilization of biomembranes. SLO serves as a diagnostic reagent for determination of anti-SLO antibody titer in streptococcal infection. Availability of highly purified SLO, however, has been limited by low yield in streptococcal culture and purification process. Present subcloning of mature-type full-length SLO gene into an expression vector having strictly controllable araBAD promoter enabled efficient production of the cytolysin. Further, anti-SLO antibody with high specificity was obtained by immunizing with purified SLO protein.
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Base de dados:
MEDLINE
Assunto principal:
Streptococcus
/
Estreptolisinas
/
Anticorpos
Limite:
Animals
Idioma:
En
Revista:
J Mol Microbiol Biotechnol
Assunto da revista:
BIOLOGIA MOLECULAR
/
BIOTECNOLOGIA
/
MICROBIOLOGIA
Ano de publicação:
2005
Tipo de documento:
Article
País de afiliação:
Japão