Separate Na,K-ATPase genes are required for otolith formation and semicircular canal development in zebrafish.
Dev Biol
; 294(1): 148-60, 2006 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-16566913
ABSTRACT
We have investigated the role of Na,K-ATPase genes in zebrafish ear development. Six Na,K-ATPase genes are differentially expressed in the developing zebrafish inner ear. Antisense morpholino knockdown of Na,K-ATPase alpha1a.1 expression blocked formation of otoliths. This effect was phenocopied by treatment of embryos with ouabain, an inhibitor of Na,K-ATPase activity. The otolith defect produced by morpholinos was rescued by microinjection of zebrafish alpha1a.1 or rat alpha1 mRNA, while the ouabain-induced defect was rescued by expression of ouabain-resistant zebrafish alpha1a.1 or rat alpha1 mRNA. Knockdown of a second zebrafish alpha subunit, alpha1a.2, disrupted development of the semicircular canals. Knockdown of Na,K-ATPase beta2b expression also caused an otolith defect, suggesting that the beta2b subunit partners with the alpha1a.1 subunit to form a Na,K-ATPase required for otolith formation. These results reveal novel roles for Na,K-ATPase genes in vestibular system development and indicate that different isoforms play distinct functional roles in formation of inner ear structures. Our results highlight zebrafish gene knockdown-mRNA rescue as an approach that can be used to dissect the functional properties of zebrafish and mammalian Na,K-ATPase genes.
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Base de dados:
MEDLINE
Assunto principal:
Membrana dos Otólitos
/
Canais Semicirculares
/
ATPase Trocadora de Sódio-Potássio
Limite:
Animals
Idioma:
En
Revista:
Dev Biol
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Estados Unidos