Cultivation and characterization of novel human group A rotaviruses with long RNA electropherotypes, subgroup II specificities, and serotype 2 VP7 genes.
J Clin Microbiol
; 29(10): 2224-7, 1991 Oct.
Article
em En
| MEDLINE
| ID: mdl-1658036
ABSTRACT
During an epidemiological study of human rotavirus infections in Bangladesh, three group A strains hybridized with a serotype 2 oligonucleotide probe, but they had long RNA electropherotypes. The three strains were collected from 8- to 20-month-old infants with acute diarrhea and moderate malnutrition. By a modified isolation procedure, two strains (T-B and T-C) were adapted in MA104 cell cultures. They were identified to be subgroup II specific by enzyme-linked immunosorbent assay with subgroup I- and II-specific monoclonal antibodies and were identified by a fluorescent focus reduction neutralization assay with hyperimmune antisera to be serotype 2 specific. Further characterization of these unusual rotavirus strains needs to be carried out.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Rotavirus
/
Proteínas do Capsídeo
/
Antígenos Virais
Limite:
Humans
/
Infant
Idioma:
En
Revista:
J Clin Microbiol
Ano de publicação:
1991
Tipo de documento:
Article