Removal of estrogenic activity of natural steroidal hormone estrone by ligninolytic enzymes from white rot fungi.
Chemosphere
; 65(1): 97-101, 2006 Sep.
Article
em En
| MEDLINE
| ID: mdl-16584756
ABSTRACT
Natural steroidal hormone estrone (E1) was treated with the white rot fungus Phanerochaete sordida YK-624 under ligninolytic condition with low-nitrogen and high-carbon culture medium. E1 decreased by 98% after 5 d of treatment and the activities of ligninolytic enzymes, manganese peroxidase (MnP) and laccase, were detected during treatment, which suggested that the disappearance of E1 is related to ligninolytic enzymes produced extracellularly by white rot fungus. Therefore, E1 was treated with MnP and laccase prepared from the culture of white rot fungi. HPLC analysis demonstrated that E1 disappeared completely in the reaction mixture after 1 h of treatment with either MnP or laccase. Using the yeast two-hybrid assay system, it was also confirmed that both enzymatic treatments completely removed the estrogenic activity of E1 after 2 h. These results strongly suggest that ligninolytic enzymes are effective in removing the estrogenic activity of E1.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Peroxidases
/
Poluentes Químicos da Água
/
Purificação da Água
/
Phanerochaete
/
Lacase
/
Estrona
Idioma:
En
Revista:
Chemosphere
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Japão