Plasma membrane destination of the classical Xenopus laevis progesterone receptor accelerates progesterone-induced oocyte maturation.
J Cell Biochem
; 99(3): 853-9, 2006 Oct 15.
Article
em En
| MEDLINE
| ID: mdl-16721828
ABSTRACT
Xenopus laevis oocyte maturation is induced by the steroid hormone progesterone through a non-genomic mechanism initiated at the cell membrane. Recently, two Xenopus oocyte progesterone receptors have been cloned; one is the classical progesterone receptor (xPR-1) involved in genomic actions and the other a putative seven-transmembrane-G-protein-couple receptor. Both receptors are postulated to be mediating the steroid-induced maturation process in the frog oocyte. In this study, we tested the hypothesis that the classical progesterone receptor, associated to the oocyte plasma membrane, is participating in the reinitiation of the cell cycle. Addition of a myristoilation and palmytoilation signal at the amino terminus of xPR-1 (mp xPR-1), increased the amount of receptor associated to the oocyte plasma membrane and most importantly, significantly potentiated progesterone-induced oocyte maturation sensitivity. These findings suggest that the classical xPR-1, located at the plasma membrane, is mediating through a non-genomic mechanism, the reinitiation of the meiotic cell cycle in the X. laevis oocyte.
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Base de dados:
MEDLINE
Assunto principal:
Oócitos
/
Progesterona
/
Receptores de Progesterona
/
Membrana Celular
/
Proteínas de Xenopus
Limite:
Animals
Idioma:
En
Revista:
J Cell Biochem
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Chile