Studies on GM-CSF DNA as an adjuvant for neutralizing Ab elicited by a DNA/MVA immunodeficiency virus vaccine.
Virology
; 352(2): 285-94, 2006 Sep 01.
Article
em En
| MEDLINE
| ID: mdl-16740288
ABSTRACT
Here, we use a vaccine consisting of DNA priming followed by MVA boosting in rhesus macaques to investigate the ability of GM-CSF DNA to serve as an adjuvant for the elicitation of neutralizing Ab against an HIV-1 Env. The trial used Gag, Pol, and Env sequences from SHIV-89.6 in the immunogens and a neutralization escape variant of SHIV-89.6, SHIV-89.6P, for challenge. Co-delivery of GM-CSF and vaccine DNAs enhanced the temporal appearance of neutralizing Ab and broadened the specificity of the neutralizing activity to include SHIV-89.6P. Two long-term SHIV-89.6 infections elicited neutralizing activity for SHIV-89.6 but not SHIV-89.6P. Studies on the avidity of the anti-Env antisera revealed that the GM-CSF-adjuvanted vaccine had elicited higher avidity Ab than the non-adjuvanted vaccine or the infection. The GM-CSF-adjuvanted group showed a trend towards better control of the challenge infection and had better control of re-emergent virus (P < 0.01) than the non-adjuvanted group.
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Base de dados:
MEDLINE
Assunto principal:
Adjuvantes Imunológicos
/
Fator Estimulador de Colônias de Granulócitos e Macrófagos
/
Vacinas contra a AIDS
/
Vacinas de DNA
Limite:
Animals
Idioma:
En
Revista:
Virology
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Estados Unidos