A dicistronic gene pair within a cluster of "EF-hand" protein genes in the genomes of Drosophila species.
Genomics
; 88(3): 347-59, 2006 Sep.
Article
em En
| MEDLINE
| ID: mdl-16750900
ABSTRACT
Androcam is a Drosophila melanogaster calmodulin-related protein that functions specifically in the testis. We show that the Acam gene is part of a cluster of three intronless genes arranged in a head-to-tail manner. The additional genes also encode calmodulin-related proteins with testis-specific transcription. Acam and the 5'-most gene (gene1) generate monocistronic transcripts. Surprisingly, the central gene (gene2) is transcribed only as a dicistronic transcript with Acam. A similar cluster is found in D. yakuba. In D. pseudoobscura, the cluster contains four genes two Acam-type genes downstream of a single gene related to both gene1 and gene2 and a fourth weakly related gene. Nevertheless, the D. pseudoobscura cluster also generates a dicistronic transcript from a gene pair analogous to the gene2-Acam pair. A cotranscribed gene1/2-Acam gene pair may be the founding feature of this locus. Although Acam protein is present in D. melanogaster and D. pseudoobscura testes, cognate proteins for the gene1/2-type ORFs are not detectable by immunoblotting and mass spectrometry techniques.
Buscar no Google
Base de dados:
MEDLINE
Assunto principal:
Proteínas de Ligação ao Cálcio
/
Família Multigênica
/
Fases de Leitura Aberta
/
Proteínas de Drosophila
/
Drosophila
/
Genoma de Inseto
Limite:
Animals
Idioma:
En
Revista:
Genomics
Assunto da revista:
GENETICA
Ano de publicação:
2006
Tipo de documento:
Article
País de afiliação:
Estados Unidos